Biofouling:
The Journal of Bioadhesion and Biofilm Research, Volume 28, Issue 9, 2012,
pages 1003-1010
Molecular
tools to track bacteria responsible for fuel deterioration and
microbiologically influenced corrosion
Joseph M. Suflita a*, Deniz F. Aktas a, Athenia L. Oldham a, Beatriz
Monica Perez-Ibarra a & Kathleen Duncan a
a The Biocorrosion Center, Institute for Energy and the Environment, University
of Oklahoma, Norman, OK, 73019, USA
Abstract
Because
the fuels themselves are not sterile, investigating the susceptibility of
various fuels to anaerobic biodegradation is not easy. Researchers obtained bacterial DNA by filtering
various fuels through a hydrophobic teflon membrane filter. Bacterial 16S rRNA genes from these
preparations were PCR amplified, cloned, and the resulting libraries sequenced
to identify the fuel-borne bacterial communities.
The most common sequence, found in algal- and camelina-based
biofuels as well as in ultra-low sulfur diesel (ULSD) and F76 diesel, was
similar to that of a Tumebacillus. The next most common sequence was similar to
Methylobacterium. Higher level
phylogenetic groups included representatives of the Firmicutes (Bacillus,
Lactobacillus and Streptococcus), several Actinobacteria, Deinococcus-Thermus,
Chloroflexi, Cyanobacteria, Bacteroidetes, Alphaproteobacteria
(Methylobacterium and Sphingomonadales), Betaproteobacteria (Oxalobacteraceae
and Burkholderiales) and Deltaproteobacteria. Both traditional and alternate fuel
formulations harbor a characteristic microflora, but these microorganisms
contributed little to the successional patterns that ultimately resulted in
fuel decomposition, sulfide formation and metal biocorrosion. The findings
illustrate the value of molecular approaches to track the fate of bacteria that
might come in contact with fuels and potentially contribute to corrosion
problems throughout the energy value chain.
Full Text Source (Subscription or Fee): http://www.tandfonline.com/doi/abs/10.1080/08927014.2012.723695
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