Degradation of Petroleum Refinery Waste by A Consortium of Hydrocarbonoclastic Bacteria

J.Trop.Life.Science, Vol 2. No 1. Jan, 2012
Degradation of Petroleum Refinery Waste by A Consortium of Hydrocarbonoclastic Bacteria
Cultured on Combinations Of Medium C:N:P Ratio
Syukria I. Zam1* and Irfan Mustafa2
siz.pku@gmail.com
1Faculty of Agriculture and Husbandry, UIN Sultan Syarif Kasim, Riau
2Biology Department, Faculty of Sciences, Brawijaya University, Malang
ABSTRACT
Bioremediation is an alternative method to treat petroleum waste using microorganism into nontoxic end product. It is relatively cheap, effective, and environmental friendly. A key factor influencing bioremediation process for petroleum refinery waste treatment is C:N:P ratio of bacterial growth medium.
The objective of this research was to obtain C:N:P ratio of Stone Mineral Salt Solution (SMSS) medium that allow optimal degradation of petroleum refinery waste by consortium of hydrocarbonoclastic bacteria. C:N:P ratio of SMSS medium was adjusted to ratio of 100:10:1, 100:10:0.5, 100:5:1, and 100:5:0.5. We demonstrate that optimal degradation of petroleum refinery waste by a consortium of hydrocarbonoclastic bacteria was achieved in SMSS medium with C:N:P ratio of 100:5:1. It allowed 66.55% degradation of total petroleum hydrocarbon (TPH) and 85.18 % decrease of chemical oxygen demand (COD) value.
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