PNAS-Proceedings of the National Academy of Sciences of the United
States of America, January 22, 2013, vol. 110 no. 4
Cyanobacterial conversion of carbon dioxide to
2,3-butanediol
John W. K. Olivera, 1, Iara M. P. Machadoa, 1,
Hisanari Yoneda a,b, and Shota Atsumia,
2
a Department of Chemistry, University of California, Davis, CA, 95616; and
b Central R&D Laboratories, New Business Development, Asahi Kasei
Corporation, Fuji, Shizuoka, 416-8501, Japan
Abstract
Although conversion of CO2 for the synthesis of
chemicals by photosynthetic organisms is an attractive target for establishing
independence from fossil reserves, synthetic pathway construction in
cyanobacteria is still in its infancy compared with model fermentative
organisms. Authors describe systematic development of the 2,3-butanediol (23BD)
biosynthetic pathway in Synechococcus elongatus PCC7942 as a model system to
establish design methods for efficient exogenous chemical production in
cyanobacteria.
They identified 23BD as a target chemical with
low host toxicity, and designed an oxygen-insensitive, cofactor-matched
biosynthetic pathway coupled with irreversible enzymatic steps to create a
driving force toward the target. Production of 23BD from CO2 reached 2.38 g/L,
a significant increase for chemical production from exogenous pathways in
cyanobacteria. Authors show that developing strong design methods can continue
to increase chemical production in cyanobacteria.
Free Full Text Source: http://www.pnas.org/content/110/4/1249.full
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