Isolation and identification of hydrocarbons degrading bacteria in soil around Shiraz Refinery

African Journal of Microbiology Research Vol. 4(19), pp. 3084-3089, 23 September, 2011
Isolation and identification of hydrocarbons degrading bacteria in soil around Shiraz Refinery
Farshid Kafilzadeh*, Parvaneh Sahragard, Hooshang Jamali and Yaghoob Tahery
Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom Iran.
For many years scientists have searched for a solution for removing petroleum hydrocarbon contaminants from soil and water. Bioremediation, the use of microorganisms, shows promise for removing crude oil pollution from contaminated sites.  The purpose of this research is to isolate and identify hydrocarbons degrading bacteria from Shiraz Refinery soil.
In this study, nutrient agar (NA) and mineral salt agar (MM2) were used as basic cultures. Using the Techniques Replica plating, we transferred colonies on NA surface to the MM2 agar. For spraying hydrocarbons on the MM2 agar surface, we used spray plating technique. Finally we have identified different bacterial genera by using biochemical tests and morphological study. Upon examination on morphological studies and biochemical tests, it is determined that these strains belong to a number of bacterial genera, which are listed.
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